Testing of parameters in cannabis for medical purposes

1. Ph.EUR 11.5 Cannabis flower (3028)

HPLC/DAD method for the identification and quantification of cannabinoids in cannabis flower (CBDA; CBD; CBN; CBNA; d9-THC; THCA-А)

Range:

Flower: (0,2÷ 32.0) %

Dried, whole or fragmented, fully developed female inflorescence of Cannabis sativa L.

  2. In-house method IR 7.2.1-37K (v.3, 03.11.2021)

HPLC method for the identification and quantification of cannabinoids in cannabis flower, oil and extract (CBDV; CBDA; CBG; CBD; THCV; CBN; d9-THC; d8-THC; CBL; CBC; THCA-А, CBGA)

Range:

Flower and oil: ≥ 0,1% w/w

Extract: ≥ 0,2% w/w

Pharmaceuticals/ cannabis flower, oil and extract

  3. EUROPEAN PHARMACOPOEIA 11.5 Loss on Drying (2.2.32)

Loss on drying

Range:

< 12%

Pharmaceuticals/ cannabis flower

  4. In-house method IR 7.2.1-45TK, v.1

Determination of terpenes content with GC/FID

(alpha-Pinene, Camphene, beta-Pinene, Myrcene, alpha-Phellandrene, Carene, alpha Terpinene, p-Cymene, D-Limonene, Eucalyptol, Ocymene, beta-Ocymene, gamma-Terpinene, Terpinolene, Fenchone, Linalol, Fenchol, Camphor, Isopulegol, Isoborneol, endo-Borneol d-L-Menthol, Terpineol, a-Terpineol, Nerol, Pulegone, Geraniol, Cedrene, trans-Caryophyllene, Farnesane, Humulene, Valencene, cis-Nerolidol, trans-Nerolidol, Guaiol, Cedrol, alpha Bisabolol)

Range:

≥ 0,05 mg/g

Pharmaceuticals/ cannabis flower